Then, membranes had been stripped, reprobed with secondary monoclonal antibodies towards the non-phosphorylated type of Akt or polyclonal antibodies to GSK3 or p65 mainly because controls of proteins loading, and detected using the Immobilon European Chemiluminescent HRP substrate package from Millipore (Billerica, MA, USA). RNA qRT-PCR and Extraction To investigate the relative manifestation of IL-12p40 … Continue reading Then, membranes had been stripped, reprobed with secondary monoclonal antibodies towards the non-phosphorylated type of Akt or polyclonal antibodies to GSK3 or p65 mainly because controls of proteins loading, and detected using the Immobilon European Chemiluminescent HRP substrate package from Millipore (Billerica, MA, USA)
Copy and paste this URL into your WordPress site to embed
Copy and paste this code into your site to embed